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Quantitative Human PLA1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX627

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Specifications
Product Description
The quantitative human PLA1 (glycosylated) sandwich ELISA kit is designed to detect human phospholipase A1 (PLA1) levels. PLA1 is an enzyme that catalyzes the hydrolysis of phospholipids at the sn-1 position, releasing fatty acids. This enzyme is involved in lipid metabolism and the generation of signaling molecules. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.097 ng/mL, which can accurately detect low concentrations of PLA1 in the sample.
Target
PLA1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.097 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Phospholipase A1
Alternate Names
PLA1; Phospholipase A1; PLA1A
Uniprot No.
N-Glycosylation Site
79, 365
Application Notes
Application
The quantitative human PLA1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PLA1 levels in biological samples. The kit is applicable in studies of lipid metabolism, inflammation, and cell signaling. It is useful in fields such as biochemistry and cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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