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Quantitative Human PRH2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX160

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Specifications
Product Description
The quantitative human PRH2 (glycosylated) sandwich ELISA kit is designed to detect human acidic salivary proline rich phosphoprotein 2 (PRH2) levels. PRH2 is a salivary protein involved in enamel formation and protection, as well as calcium binding. It plays a role in maintaining oral health and protecting teeth from demineralization. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.692 ng/mL, which can accurately detect low concentrations of PRH2 in the sample.
Target
PRH2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.692 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2.25 ng/mL-100 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Acidic salivary proline rich phosphoprotein 2
Alternate Names
PRH2; Acidic salivary proline rich phosphoprotein 2; Parotid proline-rich protein
Uniprot No.
O-Glycosylation Site
33, 38
Application Notes
Application
The quantitative human PRH2 (glycosylated) sandwich ELISA kit is utilized in research investigating oral health, dental caries, and salivary protein composition. Researchers employ this ELISA to quantify PRH2 levels, providing insights into its role in oral homeostasis and potential diagnostic applications related to salivary function.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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