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Quantitative Human PRH2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX160)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PRH2 (glycosylated) sandwich ELISA kit is designed to detect human acidic salivary proline rich phosphoprotein 2 (PRH2) levels. PRH2 is a salivary protein involved in enamel formation and protection, as well as calcium binding. It plays a role in maintaining oral health and protecting teeth from demineralization. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.692 ng/mL, which can accurately detect low concentrations of PRH2 in the sample.
Target PRH2
O-Glycosylation Site 33, 38
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.692 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.25 ng/mL-100 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Acidic salivary proline rich phosphoprotein 2
Alternate Names PRH2; Acidic salivary proline rich phosphoprotein 2; Parotid proline-rich protein
Uniprot No. P02810
Application The quantitative human PRH2 (glycosylated) sandwich ELISA kit is utilized in research investigating oral health, dental caries, and salivary protein composition. Researchers employ this ELISA to quantify PRH2 levels, providing insights into its role in oral homeostasis and potential diagnostic applications related to salivary function.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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