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Quantitative Human PSMα1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX333

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Specifications
Product Description
The quantitative human PSMα1 (glycosylated) sandwich ELISA kit is designed to detect human proteasome subunit α type 1 (PSMα1) levels. PSMα1 is a component of the 20S proteasome. The proteasome is a protein complex, which is responsible for the degradation of intracellular proteins. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.069 ng/mL, which can accurately detect low concentrations of PSMα1 in the sample.
Target
PSMα1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.069 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Proteasome subunit α type 1
Alternate Names
PSMα1; Proteasome subunit α type 1; PROS30; Multicatalytic endopeptidase complex subunit C2
Uniprot No.
O-Glycosylation Site
110
Application Notes
Application
The quantitative human PSMα1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PSMα1 levels. It is useful for studying the role of PSMα1 in proteasome functions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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