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Quantitative Human S1PR1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX294

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Specifications
Product Description
The quantitative human S1PR1 (glycosylated) sandwich ELISA kit is designed to detect human sphingosine 1 phosphate receptor 1 (S1PR1) levels. S1PR1 is a protein that functions as a G protein-coupled receptor. This receptor is activated by sphingosine 1-phosphate, which plays a role in cell migration, angiogenesis, and immune regulation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.075 ng/mL, which can accurately detect low concentrations of S1PR1 in the sample.
Target
S1PR1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.075 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sphingosine 1 phosphate receptor 1
Alternate Names
S1PR1; Sphingosine 1 phosphate receptor 1; CD363; S1P1; ECGF1; Endothelial differentiation G-protein coupled receptor 1
Uniprot No.
N-Glycosylation Site
30, 36
Application Notes
Application
The quantitative human S1PR1 (glycosylated) sandwich ELISA kit is used to measure the levels of S1PR1 in biological samples. Researchers use it in studies examining autoimmune diseases, cancer metastasis, and cardiovascular diseases. The kit is a valuable tool in pharmacological research and the development of new treatments.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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