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Quantitative Human TSPAN1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX77

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Specifications
Product Description
The quantitative human TSPAN1 (glycosylated) sandwich ELISA kit is designed to detect human tetraspanin 1 (TSPAN1) levels. TSPAN1 is a member of the tetraspanin superfamily, transmembrane proteins involved in various cellular processes, including cell adhesion, migration, and signaling by organizing membrane microdomains. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.095 ng/mL, which can accurately detect low concentrations of TSPAN1 in the sample.
Target
TSPAN1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.095 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Tetraspanin 1
Alternate Names
TSPAN1; Tetraspanin 1; NET-1; Tetraspanin TM4-C
Uniprot No.
N-Glycosylation Site
141, 154, 178, 184
Application Notes
Application
The quantitative human TSPAN1 (glycosylated) sandwich ELISA kit is valuable for research investigating TSPAN1's role in cancer metastasis, cell signaling, and immune responses. The quantitative data obtained from this kit allows for precise analysis of TSPAN1 levels, contributing to a better understanding of its function in both normal and pathological conditions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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