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Quantitative Human WIF1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX950)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human WIF1 (glycosylated) sandwich ELISA kit is designed to detect human wnt inhibitory factor 1 (WIF1) levels. WIF1 is a secreted protein that binds to Wnt proteins. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 12.535 pg/mL, which can accurately detect low concentrations of WIF1 in the sample.
Target WIF1
N-Glycosylation Site 88, 245
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 12.535 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 50 pg/mL-1500 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Wnt inhibitory factor 1
Alternate Names WIF1; Wnt inhibitory factor 1
Uniprot No. Q9Y5W5
Application The quantitative human WIF1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated WIF1 levels in biological samples. The assay is valuable for investigating the role of WIF1 in Wnt signaling regulation, development, and cancer biology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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