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Quantitative Human WIF1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX950

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Specifications
Product Description
The quantitative human WIF1 (glycosylated) sandwich ELISA kit is designed to detect human wnt inhibitory factor 1 (WIF1) levels. WIF1 is a secreted protein that binds to Wnt proteins. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 12.535 pg/mL, which can accurately detect low concentrations of WIF1 in the sample.
Target
WIF1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
12.535 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 pg/mL-1500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Wnt inhibitory factor 1
Alternate Names
WIF1; Wnt inhibitory factor 1
Uniprot No.
N-Glycosylation Site
88, 245
Application Notes
Application
The quantitative human WIF1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated WIF1 levels in biological samples. The assay is valuable for investigating the role of WIF1 in Wnt signaling regulation, development, and cancer biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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