GlycoFlux™ Human ACLY Overexpression HEK293T Cell Line (CAT#: GLJF-0925-JF490)

Description	GlycoFlux™ human ACLY overexpression HEK293T cell line is a stable cell line engineered to express ATP citrate lyase (ACLY), a tetrameric enzyme producing acetyl-CoA from citrate, essential for fatty acid and cholesterol biosynthesis as well as acetylcholine production in neurons. ACLY links glucose metabolism with lipid synthesis and epigenetic regulation. This model supports studies in cancer metabolism, neurobiology, epigenetics, and therapeutic strategies targeting acetyl-CoA metabolism.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ACLY
Full Name	Atp Citrate Lyase
Alternative Name	ACL; ATPCL; CLATP
Location	17q21.2
Gene ID	47
Summary	ATP citrate lyase is an enzyme that generates cytosolic acetyl-CoA from citrate and CoA in many tissues. It operates as a tetramer and catalyzes the conversion of citrate to acetyl-CoA and oxaloacetate, with ATP hydrolysis. Acetyl-CoA plays a role in lipogenesis, cholesterogenesis, and possibly acetylcholine biosynthesis in the nervous system.