GlycoFlux™ Human ACLY Overexpression HEK293T Cell Line

GlycoFlux™ human ACLY overexpression HEK293T cell line is a stable cell line engineered to express ATP citrate lyase (ACLY), a tetrameric enzyme producing acetyl-CoA from citrate, essential for fatty acid and cholesterol biosynthesis as well as acetylcholine production in neurons. ACLY links glucose metabolism with lipid synthesis and epigenetic regulation. This model supports studies in cancer metabolism, neurobiology, epigenetics, and therapeutic strategies targeting acetyl-CoA metabolism.

Overexpression Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293T

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

DMEM & FBS & Penicillin/Streptomycin

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ACLY

Atp Citrate Lyase

ACL; ATPCL; CLATP

17q21.2

ATP citrate lyase is an enzyme that generates cytosolic acetyl-CoA from citrate and CoA in many tissues. It operates as a tetramer and catalyzes the conversion of citrate to acetyl-CoA and oxaloacetate, with ATP hydrolysis. Acetyl-CoA plays a role in lipogenesis, cholesterogenesis, and possibly acetylcholine biosynthesis in the nervous system.