GlycoFlux™ Human ACLY Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF606)

Description	GlycoFlux™ human ACLY overexpression HepG2 cell line is a stable cell line engineered to express ATP citrate lyase (ACLY), a tetrameric enzyme producing cytosolic acetyl-CoA from citrate and CoA with ATP hydrolysis. Acetyl-CoA supports fatty acid and cholesterol biosynthesis and contributes to acetylcholine production in neurons. Multiple isoforms exist, and this model is well suited for research in lipid metabolism, cancer biology, neurochemistry, and epigenetic regulation.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ACLY
Full Name	Atp Citrate Lyase
Alternative Name	ACL; ATPCL; CLATP
Location	17q21.2
Gene ID	47
Summary	ATP citrate lyase is the major enzyme responsible for producing cytosolic acetyl-CoA in numerous tissues. It exists as a tetramer of identical subunits with a total molecular weight of about 440 kDa. This enzyme converts citrate and CoA into acetyl-CoA and oxaloacetate, coupled with the hydrolysis of ATP to ADP and phosphate. The resulting acetyl-CoA feeds into critical biosynthetic pathways, such as fatty acid and cholesterol synthesis. In the nervous system, it may contribute to acetylcholine production. Several transcript variants encoding distinct isoforms have been identified.