GlycoErase™ ACLY Knockout Glycoengineered Cell

GlycoErase™ ACLY knockout glycoengineered cell is a model system to study the role of ATP citrate lyase (ACLY), the key enzyme generating cytosolic acetyl-CoA from citrate. ACLY supports lipid and cholesterol biosynthesis and contributes to acetylcholine production in neural tissues. Knocking out ACLY enables investigation of how disrupted acetyl-CoA supply affects metabolism, membrane composition, and glycosylation, making this cell line valuable for research in metabolism, cancer, and drug discovery.

KO Cell Lines

As requested by the client.

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ACLY

Atp Citrate Lyase

ACL; ATPCL; CLATP

17q21.2

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.