Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ Lenti-ALDOA Viral Particle provides ALDOA, a class I fructose-bisphosphate aldolase catalyzing FBP cleavage to GAP and DHAP; the replication-defective construct is suited to create long-term, stably transduced mammalian cells for glycolytic flux assays, isozyme comparisons, and mutation rescue linked to GSD XII, with straightforward selection or fluorescence sorting.

Product Type

Recombinant Lentivirus

Target

ALDOA

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Insert Identity Confirmation

All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Target Information

Full Name

Aldolase, Fructose-Bisphosphate A

Alternative Name

ALDA; GSD12; HEL-S-87p

Location

16p11.2

Gene ID

226

Summary

This class I fructose-bisphosphate aldolase catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Isozymes A, B, and C are expressed at different developmental stages. Mutations cause Glycogen Storage Disease XII and are associated with cancer. Pseudogenes are found on chromosomes 3 and 10.

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GlycoFlux™ Lenti-Human ALDOA Viral Particle