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GlycoFlux™ Lenti-Human LDHA Viral Particle (CAT#: GLJF-1025-JF182)

Datasheet

SPECIFIC INQUIRY
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Titer:
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Description GlycoFlux™ Lenti-LDHA Viral Particle encodes LDHA, the A subunit of lactate dehydrogenase catalyzing pyruvate⇄lactate interconversion; deficiency can cause exertional myoglobinuria. This system enables construction of stably transduced mammalian cells for Warburg-effect modeling, metabolic flux assays, and inhibitor/variant testing, with clean integration into selection or marker-guided sorting.
Product Type Recombinant Lentivirus
Target LDHA
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name Lactate Dehydrogenase A
Alternative Name LDHM; GSD11; PIG19; HEL-S-133P
Location 11p15.1
Gene ID 3939
Summary This gene encodes the A subunit of lactate dehydrogenase, an enzyme catalyzing the conversion of pyruvate to lactate. It is mainly expressed in skeletal muscle and mutations lead to exertional myoglobinuria. Pseudogenes of this gene exist.
For Research Use Only.
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