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GlycoFlux™ Lenti-Human POFUT2 Viral Particle (CAT#: GLJF-1025-JF91)

Datasheet

SPECIFIC INQUIRY
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Titer:
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Description GlycoFlux™ Lenti-POFUT2 Viral Particle delivers POFUT2, adding O-fucose to Ser/Thr within thrombospondin type-1 repeats (TSRs) and related cystine-knot motifs to modulate protein folding and activity. Purpose-built to generate stably transduced mammalian cells, this system enables analysis of TSR-dependent secretion/quality control, rescue of loss-of-function backgrounds, and mechanism-focused screening with straightforward selection or FACS sorting.
Product Type Recombinant Lentivirus
Target POFUT2
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name Protein O-fucosyltransferase 2
Alternative Name C21orf80; FUT13; KIAA0958
Location 21q22.3
Gene ID 23275
Summary Adds O-fucose to serine/threonine in cystine knot motifs within EGF-like or thrombospondin type-1 repeats, modulating protein activity.
For Research Use Only.
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