GlycoFlux™ Lenti-Human POFUT2 Viral Particle

GlycoFlux™ Lenti-POFUT2 Viral Particle delivers POFUT2, adding O-fucose to Ser/Thr within thrombospondin type-1 repeats (TSRs) and related cystine-knot motifs to modulate protein folding and activity. Purpose-built to generate stably transduced mammalian cells, this system enables analysis of TSR-dependent secretion/quality control, rescue of loss-of-function backgrounds, and mechanism-focused screening with straightforward selection or FACS sorting.

Recombinant Lentivirus

POFUT2

Human

EF1a

Lentivirus

HEK293T

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipped on dry ice with temperature held at or below -80°C.

Store at -80°C to maintain their titer and stability.

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

qPCR

Negative

Sterility testing confirmed no microbial contamination.

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Protein O-fucosyltransferase 2

C21orf80; FUT13; KIAA0958

21q22.3

Adds O-fucose to serine/threonine in cystine knot motifs within EGF-like or thrombospondin type-1 repeats, modulating protein activity.