Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ MMLV-PHKG2 Viral Particle delivers PHKG2, the hepatic γ catalytic isoform (GSD IXc gene); configured to establish stable expression in mammalian cells, it enables active-site variant analysis and liver-specific glycogen mobilization studies.

Product Type

Recombinant MMLV Retrovirus

Target

PHKG2

Species

Human

Promotor

MMLV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Insert Identity Confirmation

Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Target Information

Full Name

Phosphorylase Kinase Catalytic Subunit Gamma 2

Alternative Name

GSD9C

Location

16p11.2

Gene ID

5261

Summary

The hepatic gamma isoform of phosphorylase kinase is encoded by this gene, part of a 16-subunit complex. The gamma subunit contains the active site, with alpha and beta serving regulatory functions. Defects cause glycogen storage disease type 9C (autosomal liver glycogenosis). Several splice isoforms have been described.

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GlycoFlux™ MMLV-Human PHKG2 Viral Particle