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GlycoFlux™ MSCV-Human PPP1R12A Viral Particle (CAT#: GLJF-1025-JF726)

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Description GlycoFlux™ MSCV-PPP1R12A Viral Particle provides stable MYPT1 expression to direct PP1 toward myosin light-chain substrates and reshape actomyosin tone. Ideal for smooth-muscle and adhesion studies; measure MLC phosphorylation, traction forces, and RhoA/ROCK signaling interactions.
Product Type Recombinant MSCV Retrovirus
Target PPP1R12A
Species Human
Promotor MSCV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.
Shipping Dispatched on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to preserve titer and stability.
Handling Notes Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.
Full Name Protein Phosphatase 1 Regulatory Subunit 12A
Alternative Name MBS; GUBS; M130; MYPT1
Location 12q21.2-q21.31
Gene ID 4659
Summary Myosin phosphatase target subunit 1 (MBS) is a component of myosin phosphatase, regulating actin-myosin interactions downstream of the small GTPase Rho. Active RhoA binds MBS, influencing myosin light chain (MLC) phosphorylation via Rho-kinase. This phosphorylation reduces myosin phosphatase activity, promoting smooth muscle contraction and actin-myosin association in nonmuscle cells. Overexpression of RhoA or its active form increases MBS and MLC phosphorylation. Several isoforms result from alternative splicing.
For Research Use Only.
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