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| Description | GlycoFlux™ MMLV-PPP1R12A Viral Particle delivers PPP1R12A (MYPT1), the myosin-phosphatase targeting subunit that directs PP1 to myosin light chain; this system creates long-term stable mammalian models for smooth-muscle contractility, cytoskeletal dynamics, and Rho/PKA pathway integration. |
| Product Type | Recombinant MMLV Retrovirus |
| Target | PPP1R12A |
| Species | Human |
| Promotor | MMLV 5' LTR promoter |
| Packaging System | Retrovirus |
| Packaging Cell | HEK293T |
| Application | This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research. |
| Shipping | Shipped on dry ice with temperature held at or below -80°C. |
| Storage | Store at -80°C to maintain their titer and stability. |
| Handling Notes | Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection. |
| Product Disclaimer | These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application. |
| Titer Assay | qPCR |
| Mycoplasma Test | Negative |
| Sterility | Sterility testing confirmed no microbial contamination. |
| Transduction Evaluation | On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function. |
| Insert Identity Confirmation | Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration. |
| Full Name | Protein Phosphatase 1 Regulatory Subunit 12A |
| Alternative Name | MBS; GUBS; M130; MYPT1 |
| Location | 12q21.2-q21.31 |
| Gene ID | 4659 |
| Summary | Myosin phosphatase target subunit 1 (MYPT1) is a regulatory subunit of myosin phosphatase, involved in smooth muscle contraction and actin-myosin interactions. Several isoforms have been identified. |