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Quantitative Human 5HTR2B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX407

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Specifications
Product Description
The quantitative human 5HTR2B (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 2B (5HTR2B) levels. 5HTR2B is a G protein-coupled receptor that binds serotonin, which is found in various tissues, including the brain, heart, and blood vessels. 5HTR2B activation is involved in several physiological processes, including mood regulation, cardiovascular functions, and cell growth. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.094 ng/mL, which can accurately detect low concentrations of 5HTR2B in the sample.
Target
5HTR2B
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.094 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
5-Hydroxytryptamine receptor 2B
Alternate Names
5HTR2B; 5-Hydroxytryptamine receptor 2B
Uniprot No.
N-Glycosylation Site
30
Application Notes
Application
The quantitative human 5HTR2B (glycosylated) sandwich ELISA kit is used to measure glycosylated 5HTR2B levels in samples. This measurement is valuable in research focusing on serotonin signaling and related disorders. The kit is applied in studies of psychiatric conditions, cardiovascular diseases, and cancer, where 5HTR2B expression may play a role.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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