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Quantitative Human ABCC5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX47

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Specifications
Product Description
The quantitative human ABCC5 (glycosylated) sandwich ELISA kit is designed to detect human ATP binding cassette transporter C5 (ABCC5) levels. ABCC5 is a membrane protein that functions in the transport of various molecules, including cyclic nucleotides and certain drugs, across cell membranes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.129 ng/mL, which can accurately detect low concentrations of ABCC5 in the sample.
Target
ABCC5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.129 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
ATP binding cassette transporter C5
Alternate Names
ABCC5; ATP binding cassette transporter C5; ABC33; MOATC; MRP5; Multidrug resistance-associated protein 5; Multi-specific organic anion transporter C
Uniprot No.
N-Glycosylation Site
494, 636, 684, 890, 897, 1044, 1329, 1417
Application Notes
Application
The quantitative human ABCC5 (glycosylated) sandwich ELISA kit is utilized for the in vitro quantification of glycosylated human ABCC5 in biological samples. This assay employs a sandwich ELISA technique, enabling researchers to determine ABCC5 protein levels, which is valuable in studies concerning drug resistance, cellular signaling pathways, and the transport of physiological substrates. This kit aids in research exploring the roles of ABCC5 in various physiological and pathological processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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