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Quantitative Human ABCC8 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX543

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Specifications
Product Description
The quantitative human ABCC8 (glycosylated) sandwich ELISA kit is designed to detect human ATP binding cassette transporter C8 (ABCC8) levels. ABCC8 is a protein that forms a subunit of the ATP-sensitive potassium (KATP) channel. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.104 ng/mL, which can accurately detect low concentrations of ABCC8 in the sample.
Target
ABCC8
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.104 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
ATP binding cassette transporter C8
Alternate Names
ABCC8; ATP binding cassette transporter C8; MRP8; HHF1; SUR1; TNDM2
Uniprot No.
N-Glycosylation Site
10, 1049
Application Notes
Application
The quantitative human ABCC8 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology, endocrinology, and diabetes, particularly in studies of KATP channels and insulin secretion. Researchers use it to measure ABCC8 levels in pancreatic tissue and other samples. The kit has applications in studies of diabetes and metabolic disorders.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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