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Quantitative Human ARSA (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX251

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Specifications
Product Description
The quantitative human ARSA (glycosylated) sandwich ELISA kit is designed to detect human arylsulfatase A (ARSA) levels. ARSA is a lysosomal enzyme that hydrolyzes sulfatide lipids, playing a critical role in the degradation of sulfated glycosphingolipids within the nervous system. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.074 ng/mL, which can accurately detect low concentrations of ARSA in the sample.
Target
ARSA
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.074 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Arylsulfatase A
Alternate Names
ARSA; Arylsulfatase A; MLD; ASA; Metachromatic leucodystrophy
Uniprot No.
N-Glycosylation Site
158, 184, 350
Application Notes
Application
The quantitative human ARSA (glycosylated) sandwich ELISA kit is widely used to measure the levels of glycosylated ARSA in biological samples, enabling research into metachromatic leukodystrophy (MLD) and other lysosomal storage diseases. This kit allows researchers to quantify ARSA levels, providing valuable data for diagnosing and studying the progression of these conditions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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