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Quantitative Human ARSA (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX251)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ARSA (glycosylated) sandwich ELISA kit is designed to detect human arylsulfatase A (ARSA) levels. ARSA is a lysosomal enzyme that hydrolyzes sulfatide lipids, playing a critical role in the degradation of sulfated glycosphingolipids within the nervous system. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.074 ng/mL, which can accurately detect low concentrations of ARSA in the sample.
Target ARSA
N-Glycosylation Site 158, 184, 350
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.074 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Arylsulfatase A
Alternate Names ARSA; Arylsulfatase A; MLD; ASA; Metachromatic leucodystrophy
Uniprot No. P15289
Application The quantitative human ARSA (glycosylated) sandwich ELISA kit is widely used to measure the levels of glycosylated ARSA in biological samples, enabling research into metachromatic leukodystrophy (MLD) and other lysosomal storage diseases. This kit allows researchers to quantify ARSA levels, providing valuable data for diagnosing and studying the progression of these conditions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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