Quantitative Human ATP4β (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX448)

Name: Quantitative Human ATP4β (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX448
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human ATP4β (glycosylated) sandwich ELISA kit is designed to detect human ATPase, β polypeptide (ATP4β) levels. ATP4β is a subunit of the H+/K+-ATPase enzyme. This enzyme is found in the stomach and is responsible for secreting gastric acid. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.093 ng/mL, which can accurately detect low concentrations of ATP4β in the sample.
Target	ATP4β
N-Glycosylation Site	99, 103, 130, 146, 161, 193, 222
Sample Types	Tissue homogenates, cell lysates, serum, plasma
Sample Volume	100 μL
Sensitivity	0.093 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	1 ng/mL-10 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Application	The quantitative human ATP4β (glycosylated) sandwich ELISA kit is designed to measure glycosylated ATP4β levels in samples. This measurement is valuable in research related to gastric acid secretion. The kit is applied in studies of conditions affecting the stomach, which helps in understanding the role of ATP4β.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-95%; Plasma sample: n=4, 80-95%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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