Quantitative Human ATP4β (Glycosylated) Sandwich ELISA Kit

The quantitative human ATP4β (glycosylated) sandwich ELISA kit is designed to detect human ATPase, β polypeptide (ATP4β) levels. ATP4β is a subunit of the H+/K+-ATPase enzyme. This enzyme is found in the stomach and is responsible for secreting gastric acid. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.093 ng/mL, which can accurately detect low concentrations of ATP4β in the sample.

ATP4β

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.093 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

ATPase, β polypeptide

ATP4β; ATPase, β polypeptide

99, 103, 130, 146, 161, 193, 222

The quantitative human ATP4β (glycosylated) sandwich ELISA kit is designed to measure glycosylated ATP4β levels in samples. This measurement is valuable in research related to gastric acid secretion. The kit is applied in studies of conditions affecting the stomach, which helps in understanding the role of ATP4β.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-95%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.