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Quantitative Human COL1α1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX149)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL1α1 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 1 α1 (COL1α1) levels. COL1α1 is a major component of the extracellular matrix, forming the fibrillar collagen that provides structural support to various tissues, including skin, bone, and tendons. Its proper function is essential for tissue integrity and repair. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.281 ng/mL, which can accurately detect low concentrations of COL1α1 in the sample.
Target COL1α1
N-Glycosylation Site 1365
O-Glycosylation Site 265, 1108
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.281 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.55 ng/mL-50 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type 1 α1
Alternate Names COL1α1; Collagen type 1 α1; COL1-A1; Collagen α1(I) chain
Uniprot No. P02452
Application The quantitative human COL1α1 (glycosylated) sandwich ELISA kit is utilized in research investigating fibrosis, wound healing, and bone metabolism, as well as in studies related to connective tissue disorders. Researchers employ this ELISA to quantify COL1α1 levels, providing insights into its role in tissue remodeling and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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