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Quantitative Human CRIM1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX864

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Specifications
Product Description
The quantitative human CRIM1 (glycosylated) sandwich ELISA kit is designed to detect human cysteine rich transmembrane bmp regulator 1 (CRIM1) levels. CRIM1 is a transmembrane protein that modulates bone morphogenetic protein (BMP) signaling. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.132 ng/mL, which can accurately detect low concentrations of CRIM1 in the sample.
Target
CRIM1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.132 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Cysteine rich transmembrane BMP regulator 1
Alternate Names
CRIM1; Cysteine rich transmembrane BMP regulator 1
Uniprot No.
N-Glycosylation Site
71, 113, 330, 474, 746
Application Notes
Application
The quantitative human CRIM1 (glycosylated) sandwich ELISA kit is used to determine CRIM1 levels in biological samples. This kit is applicable in studies examining BMP signaling and birth defects. It is also relevant to studies investigating the role of CRIM1 in regulating growth factor activity during development and its involvement in the pathogenesis of developmental abnormalities.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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