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Quantitative Human ELA3A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX211

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Specifications
Product Description
The quantitative human ELA3A (glycosylated) sandwich ELISA kit is designed to detect human elastase 3A (ELA3A) levels. ELA3A is a serine protease, primarily produced in the pancreas, that plays a role in the digestion of proteins, including elastin. It contributes to the breakdown of dietary proteins and may also have roles in tissue remodeling. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 14.924 pg/mL, which can accurately detect low concentrations of ELA3A in the sample.
Target
ELA3A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
14.924 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
35 pg/mL-1800 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Elastase 3A
Alternate Names
ELA3A; Elastase 3A; CELA3A; ELA3; Chymotrypsin-like elastase family member 3A
Uniprot No.
N-Glycosylation Site
114
Application Notes
Application
The quantitative human ELA3A (glycosylated) sandwich ELISA kit is valuable in research investigating pancreatic function, digestive disorders, and conditions involving extracellular matrix degradation. Researchers utilize this ELISA to quantify ELA3A levels, providing insights into pancreatic enzyme activity and potential diagnostic applications related to digestive health and tissue remodeling.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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