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Quantitative Human ELA3A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX211)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ELA3A (glycosylated) sandwich ELISA kit is designed to detect human elastase 3A (ELA3A) levels. ELA3A is a serine protease, primarily produced in the pancreas, that plays a role in the digestion of proteins, including elastin. It contributes to the breakdown of dietary proteins and may also have roles in tissue remodeling. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 14.924 pg/mL, which can accurately detect low concentrations of ELA3A in the sample.
Target ELA3A
N-Glycosylation Site 114
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 14.924 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 35 pg/mL-1800 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Elastase 3A
Alternate Names ELA3A; Elastase 3A; CELA3A; ELA3; Chymotrypsin-like elastase family member 3A
Uniprot No. P09093
Application The quantitative human ELA3A (glycosylated) sandwich ELISA kit is valuable in research investigating pancreatic function, digestive disorders, and conditions involving extracellular matrix degradation. Researchers utilize this ELISA to quantify ELA3A levels, providing insights into pancreatic enzyme activity and potential diagnostic applications related to digestive health and tissue remodeling.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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