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Quantitative Human EPHA3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX355

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Specifications
Product Description
The quantitative human EPHA3 (glycosylated) sandwich ELISA kit is designed to detect human ephrin type A receptor 3 (EPHA3) levels. EPHA3 is a receptor tyrosine kinase, which binds to ephrin-A ligands. EPHA3 plays a role in cell migration. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.121 ng/mL, which can accurately detect low concentrations of EPHA3 in the sample.
Target
EPHA3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.121 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Ephrin type A receptor 3
Alternate Names
EPHA3; Ephrin type A receptor 3; ETK1; HEK4; Human embryo kinase; Tyrosine-protein kinase receptor ETK1
Uniprot No.
N-Glycosylation Site
232, 337, 391, 404, 493
Application Notes
Application
The quantitative human EPHA3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated EPHA3 levels. It is useful for studying the role of EPHA3 in tumor cell migration.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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