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Quantitative Human EPHA3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX355)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human EPHA3 (glycosylated) sandwich ELISA kit is designed to detect human ephrin type A receptor 3 (EPHA3) levels. EPHA3 is a receptor tyrosine kinase, which binds to ephrin-A ligands. EPHA3 plays a role in cell migration. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.121 ng/mL, which can accurately detect low concentrations of EPHA3 in the sample.
Target EPHA3
N-Glycosylation Site 232, 337, 391, 404, 493
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.121 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Ephrin type A receptor 3
Alternate Names EPHA3; Ephrin type A receptor 3; ETK1; HEK4; Human embryo kinase; Tyrosine-protein kinase receptor ETK1
Uniprot No. P29320
Application The quantitative human EPHA3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated EPHA3 levels. It is useful for studying the role of EPHA3 in tumor cell migration.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-90%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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