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Quantitative Human FCN1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX20

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Specifications
Product Description
The quantitative human FCN1 (glycosylated) sandwich ELISA kit is designed to detect human ficolin 1 (FCN1) levels. FCN1 is a pattern recognition molecule of the lectin pathway of complement activation, playing a role in innate immunity by recognizing and binding to microbial ligands. It contributes to host defense by initiating complement-mediated opsonization and phagocytosis. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 1.315 ng/mL, which can accurately detect low concentrations of FCN1 in the sample.
Target
FCN1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
1.315 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
5 ng/mL-100 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Ficolin 1
Alternate Names
FCN1; Ficolin 1; FCNM; Ficolin-alpha; Ficolin-A
Uniprot No.
N-Glycosylation Site
305
Application Notes
Application
The quantitative human FCN1 (glycosylated) sandwich ELISA kit is valuable for researchers investigating infectious diseases, autoimmune disorders, and inflammatory conditions, where FCN1 plays a role in immune responses. By quantifying FCN1, researchers can gain insights into its involvement in disease pathogenesis and explore potential diagnostic or therapeutic applications related to complement activation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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