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Quantitative Human FZD6 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX69

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Specifications
Product Description
The quantitative human FZD6 (glycosylated) sandwich ELISA kit is designed to detect human frizzled homolog 6 (FZD6) levels. FZD6 is a member of the Frizzled family of G protein-coupled receptors, crucial in Wnt signaling pathways that regulate cell fate, proliferation, and tissue polarity during development. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.153 ng/mL, which can accurately detect low concentrations of FZD6 in the sample.
Target
FZD6
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.153 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Frizzled homolog 6
Alternate Names
FZD6; Frizzled homolog 6
Uniprot No.
N-Glycosylation Site
38, 352
Application Notes
Application
The quantitative human FZD6 (glycosylated) sandwich ELISA kit is designed to accurately measure glycosylated FZD6 levels in biological samples. This is vital for research exploring FZD6's role in developmental processes, cancer biology, and other Wnt-mediated signaling pathways.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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