Quantitative Human GIF (Glycosylated) Competitive ELISA Kit

The quantitative human GIF (glycosylated) competitive ELISA kit is designed to detect human gastric intrinsic factor (GIF) levels. GIF is secreted by parietal cells in the stomach. GIF binds to vitamin B12, which facilitates the absorption of vitamin B12 in the small intestine. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 1.103 ng/mL, which can accurately detect low concentrations of GIF in the sample.

GIF

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Serum, plasma

100 μL

1.103 ng/mL

Quantitative competitive ELISA

5.25 ng/mL-150 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Gastric intrinsic factor

GIF; Gastric intrinsic factor; TCN3; IF; INF; Transcobalamin III

311, 330, 334, 413

The quantitative human GIF (glycosylated) competitive ELISA kit is designed for the quantitative measurement of glycosylated GIF levels. It is useful for studying the role of GIF in vitamin B12 absorption.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.