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Quantitative Human GIF (Glycosylated) Competitive ELISA Kit (CAT#: GLJF-0525-HX340)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human GIF (glycosylated) competitive ELISA kit is designed to detect human gastric intrinsic factor (GIF) levels. GIF is secreted by parietal cells in the stomach. GIF binds to vitamin B12, which facilitates the absorption of vitamin B12 in the small intestine. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 1.103 ng/mL, which can accurately detect low concentrations of GIF in the sample.
Target GIF
N-Glycosylation Site 311, 330, 334, 413
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 1.103 ng/mL
Detection Principle Quantitative competitive ELISA
Detection Range 5.25 ng/mL-150 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Gastric intrinsic factor
Alternate Names GIF; Gastric intrinsic factor; TCN3; IF; INF; Transcobalamin III
Uniprot No. P27352
Application The quantitative human GIF (glycosylated) competitive ELISA kit is designed for the quantitative measurement of glycosylated GIF levels. It is useful for studying the role of GIF in vitamin B12 absorption.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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