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Quantitative Human GLT25D2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX683

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Specifications
Product Description
The quantitative human GLT25D2 (glycosylated) sandwich ELISA kit is designed to detect human glycosyltransferase 25 domain containing protein 2 (GLT25D2) levels. GLT25D2 is an enzyme, which plays a role in glycosylation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.065 ng/mL, which can accurately detect low concentrations of GLT25D2 in the sample.
Target
GLT25D2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.065 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glycosyltransferase 25 domain containing protein 2
Alternate Names
GLT25D2; Glycosyltransferase 25 domain containing protein 2; Procollagen galactosyltransferase 2; Hydroxylysine galactosyltransferase 2
Uniprot No.
N-Glycosylation Site
97, 185, 382, 580
Application Notes
Application
The quantitative human GLT25D2 (glycosylated) sandwich ELISA kit is used to determine GLT25D2 concentrations in biological specimens. This measurement is crucial for studies investigating glycosylation processes. It also contributes to understanding the role of GLT25D2 in carbohydrate metabolism. Furthermore, it is valuable in research exploring the role of glycosylation in various biological systems.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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