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Quantitative Human GPER (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX759

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Specifications
Product Description
The quantitative human GPER (glycosylated) sandwich ELISA kit is designed to detect human g protein coupled estrogen receptor 1 (GPER) levels. GPER is a receptor for estrogen that is also known as GPR30. GPER mediates some of the effects of estrogen. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.134 ng/mL, which can accurately detect low concentrations of GPER in the sample.
Target
GPER
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.134 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
G protein coupled estrogen receptor 1
Alternate Names
GPER; G protein coupled estrogen receptor 1; GPR30; CMKRL2; Chemoattractant receptor-like 2
Uniprot No.
N-Glycosylation Site
25, 32, 44
Application Notes
Application
The quantitative human GPER (glycosylated) sandwich ELISA kit is useful for measuring GPER levels in the samples. This measurement is relevant to research on estrogen signaling and hormone action. The kit also has potential applications in investigations of endocrine-related conditions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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