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Quantitative Human GUSβ (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX198)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human GUSβ (glycosylated) sandwich ELISA kit is designed to detect human glucuronidase β (GUSβ) levels. GUSβ is a lysosomal enzyme that hydrolyzes β-D-glucuronides, playing a role in the degradation of glycosaminoglycans and other complex carbohydrates. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.112 ng/mL, which can accurately detect low concentrations of GUSβ in the sample.
Target GUSβ
N-Glycosylation Site 173, 272, 420, 631
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.112 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Glucuronidase β
Alternate Names GUSβ; Glucuronidase β; GUS-B; MPS7
Uniprot No. P08236
Application The quantitative human GUSβ (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for diagnosing and monitoring mucopolysaccharidosis type VII, as well as investigating the role of GUSβ in other metabolic disorders. Researchers employ this ELISA to quantify GUSβ levels, providing insights into its activity and potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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