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Quantitative Human GUSβ (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX198

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Specifications
Product Description
The quantitative human GUSβ (glycosylated) sandwich ELISA kit is designed to detect human glucuronidase β (GUSβ) levels. GUSβ is a lysosomal enzyme that hydrolyzes β-D-glucuronides, playing a role in the degradation of glycosaminoglycans and other complex carbohydrates. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.112 ng/mL, which can accurately detect low concentrations of GUSβ in the sample.
Target
GUSβ
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
0.112 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glucuronidase β
Alternate Names
GUSβ; Glucuronidase β; GUS-B; MPS7
Uniprot No.
N-Glycosylation Site
173, 272, 420, 631
Application Notes
Application
The quantitative human GUSβ (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for diagnosing and monitoring mucopolysaccharidosis type VII, as well as investigating the role of GUSβ in other metabolic disorders. Researchers employ this ELISA to quantify GUSβ levels, providing insights into its activity and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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