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Quantitative Human IHH (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX592

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Specifications
Product Description
The quantitative human IHH (glycosylated) sandwich ELISA kit is designed to detect human hedgehog homolog (IHH) levels. IHH is a signaling molecule, which plays a crucial role in embryonic development. IHH is involved in the formation of various tissues and organs. It is particularly important in skeletal development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.065 ng/mL, which can accurately detect low concentrations of IHH in the sample.
Target
IHH
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.065 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Hedgehog homolog
Alternate Names
IHH; Hedgehog homolog; HHG2
Uniprot No.
N-Glycosylation Site
282
Application Notes
Application
The quantitative human IHH (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of IHH levels in biological specimens. This measurement is crucial for studies investigating embryonic development. It also contributes to understanding the role of IHH in skeletal formation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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