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Quantitative Human IHH (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX592)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human IHH (glycosylated) sandwich ELISA kit is designed to detect human hedgehog homolog (IHH) levels. IHH is a signaling molecule, which plays a crucial role in embryonic development. IHH is involved in the formation of various tissues and organs. It is particularly important in skeletal development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.065 ng/mL, which can accurately detect low concentrations of IHH in the sample.
Target IHH
N-Glycosylation Site 282
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.065 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Hedgehog homolog
Alternate Names IHH; Hedgehog homolog; HHG2
Uniprot No. Q14623
Application The quantitative human IHH (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of IHH levels in biological specimens. This measurement is crucial for studies investigating embryonic development. It also contributes to understanding the role of IHH in skeletal formation.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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