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Quantitative Human ITGα5 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX205)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ITGα5 (glycosylated) sandwich ELISA kit is designed to detect human integrin α5 (ITGα5) levels. ITGα5 is a subunit of the α5β1 integrin, a cell adhesion receptor that binds fibronectin, playing a crucial role in cell adhesion, migration, and proliferation. It is involved in various processes, including wound healing and tumor metastasis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.094 ng/mL, which can accurately detect low concentrations of ITGα5 in the sample.
Target ITGα5
N-Glycosylation Site 84, 182, 297, 307, 316, 524, 530, 593, 609, 675, 712, 724, 773, 868
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.094 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Integrin α5
Alternate Names ITGα5; Integrin α5; CD49-E; ITG-A5; CD49 antigen-like family member E
Uniprot No. P08648
Application The quantitative human ITGα5 (glycosylated) sandwich ELISA kit is valuable in research investigating cancer biology, fibrosis, and wound healing, where ITGα5 expression and function are relevant. Researchers utilize this ELISA to quantify ITGα5 levels, providing insights into its role in cell-matrix interactions and potential therapeutic targeting.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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