Quantitative Human MHCA (Glycosylated) Sandwich ELISA Kit

The quantitative human MHCA (glycosylated) sandwich ELISA kit is designed to detect human major histocompatibility complex class 1A (MHCA) levels. MHCA refers to the classical MHC class I molecules, primarily HLA-A, which are essential for presenting intracellular peptides to CD8+ T cells, initiating cytotoxic T cell responses. It is crucial for immune surveillance and recognition of infected or cancerous cells. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.154 ng/mL, which can accurately detect low concentrations of MHCA in the sample.

MHCA

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.154 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-20 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Major histocompatibility complex class 1A

MHCA; Major histocompatibility complex class 1A; HLAA; Leukocyte antigen A

110

The quantitative human MHCA (glycosylated) sandwich ELISA kit is valuable in research investigating transplantation, autoimmune diseases, and cancer immunotherapy, where HLA-A expression and function are critical.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.