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Quantitative Human MTNR1B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX437

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Specifications
Product Description
The quantitative human MTNR1B (glycosylated) sandwich ELISA kit is designed to detect human melatonin receptor 1B (MTNR1B) levels. MTNR1B is a G protein-coupled receptor that binds melatonin, which is involved in mediating the effects of melatonin. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.060 ng/mL, which can accurately detect low concentrations of MTNR1B in the sample.
Target
MTNR1B
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.060 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Melatonin receptor 1B
Alternate Names
MTNR1B; Melatonin receptor 1B; MT2
Uniprot No.
N-Glycosylation Site
4
Application Notes
Application
The quantitative human MTNR1B (glycosylated) sandwich ELISA kit is used to measure glycosylated MTNR1B levels in samples. This measurement is important in research related to sleep and circadian rhythms. The kit is applied in studies of conditions such as sleep disorders, which helps in understanding the role of MTNR1B.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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