Quantitative Human NEF3 (Glycosylated) Sandwich ELISA Kit

The quantitative human NEF3 (glycosylated) sandwich ELISA kit is designed to detect human neurofilament 3 (NEF3) levels. NEF3 is a type III intermediate filament protein primarily found in peripheral neurons, contributing to axonal structure and function. It plays a role in neuronal development and regeneration. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.147 ng/mL, which can accurately detect low concentrations of NEF3 in the sample.

NEF3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.147 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Neurofilament 3

NEF3; Neurofilament 3; NEFM; NFM; Neurofilament triplet M protein

47, 431

The quantitative human NEF3 (glycosylated) sandwich ELISA kit is valuable in research investigating peripheral neuropathies, neuronal injury, and neurodegenerative diseases affecting peripheral neurons. Researchers utilize this ELISA to quantify NEF3 levels, providing insights into axonal damage and potential diagnostic or therapeutic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.