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Quantitative Human NEF3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX188)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NEF3 (glycosylated) sandwich ELISA kit is designed to detect human neurofilament 3 (NEF3) levels. NEF3 is a type III intermediate filament protein primarily found in peripheral neurons, contributing to axonal structure and function. It plays a role in neuronal development and regeneration. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.147 ng/mL, which can accurately detect low concentrations of NEF3 in the sample.
Target NEF3
O-Glycosylation Site 47, 431
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.147 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Neurofilament 3
Alternate Names NEF3; Neurofilament 3; NEFM; NFM; Neurofilament triplet M protein
Uniprot No. P07197
Application The quantitative human NEF3 (glycosylated) sandwich ELISA kit is valuable in research investigating peripheral neuropathies, neuronal injury, and neurodegenerative diseases affecting peripheral neurons. Researchers utilize this ELISA to quantify NEF3 levels, providing insights into axonal damage and potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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