Quantitative Human NKCC1 (Glycosylated) Sandwich ELISA Kit

The quantitative human NKCC1 (glycosylated) sandwich ELISA kit is designed to detect human Na-K-Cl cotransporter 1 (NKCC1) levels. NKCC1 is a membrane protein that transports sodium, potassium, and chloride ions across cell membranes. It plays a role in regulating cell volume. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.205 ng/mL, which can accurately detect low concentrations of NKCC1 in the sample.

NKCC1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.205 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Na-K-Cl cotransporter 1

NKCC1; Na-K-Cl cotransporter 1; BSC; KILQS; SLC12A2

553, 562

The quantitative human NKCC1 (glycosylated) sandwich ELISA kit is designed to measure glycosylated NKCC1 levels in samples. This measurement is valuable in research related to ion transport. The kit is applied in studies of cell volume regulation, which helps in understanding the role of NKCC1.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.