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Quantitative Human NKCC1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX470)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NKCC1 (glycosylated) sandwich ELISA kit is designed to detect human Na-K-Cl cotransporter 1 (NKCC1) levels. NKCC1 is a membrane protein that transports sodium, potassium, and chloride ions across cell membranes. It plays a role in regulating cell volume. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.205 ng/mL, which can accurately detect low concentrations of NKCC1 in the sample.
Target NKCC1
N-Glycosylation Site 553, 562
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.205 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Na-K-Cl cotransporter 1
Alternate Names NKCC1; Na-K-Cl cotransporter 1; BSC; KILQS; SLC12A2
Uniprot No. P55011
Application The quantitative human NKCC1 (glycosylated) sandwich ELISA kit is designed to measure glycosylated NKCC1 levels in samples. This measurement is valuable in research related to ion transport. The kit is applied in studies of cell volume regulation, which helps in understanding the role of NKCC1.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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