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Quantitative Human NT4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX377

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Specifications
Product Description
The quantitative human NT4 (glycosylated) sandwich ELISA kit is designed to detect human neurotrophin 4 (NT4) levels. NT4 is a growth factor, which belongs to the neurotrophin family. NT4 promotes the survival and differentiation of neurons. It plays a role in nervous system development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of NT4 in the sample.
Target
NT4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Neurotrophin 4
Alternate Names
NT4; Neurotrophin 4; NTF4; Neurotrophic factor 4
Uniprot No.
N-Glycosylation Site
76
Application Notes
Application
The quantitative human NT4 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated NT4 levels. It is useful for studying the role of NT4 in supporting nerve cell development.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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