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Quantitative Human PDIA2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX555

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Specifications
Product Description
The quantitative human PDIA2 (glycosylated) sandwich ELISA kit is designed to detect human protein disulfide isomerase A2 (PDIA2) levels. PDIA2 is an enzyme that resides in the endoplasmic reticulum (ER), which catalyzes the formation and breakage of disulfide bonds in proteins. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.119 ng/mL, which can accurately detect low concentrations of PDIA2 in the sample.
Target
PDIA2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.119 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Protein disulfide isomerase A2
Alternate Names
PDIA2; Protein disulfide isomerase A2; PDA2, PDIR; Protein disulfide isomerase-associated 2; Pancreas-specific protein disulfide isomerase
Uniprot No.
N-Glycosylation Site
127, 284, 516
Application Notes
Application
The quantitative human PDIA2 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and biochemistry, particularly in studies of protein folding and ER stress. Researchers use it to measure PDIA2 levels in cells and tissues. The kit has applications in studies of protein folding and cellular stress responses.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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