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Quantitative Human PDIA2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX555)

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1×48 T
1×96 T

Product Description The quantitative human PDIA2 (glycosylated) sandwich ELISA kit is designed to detect human protein disulfide isomerase A2 (PDIA2) levels. PDIA2 is an enzyme that resides in the endoplasmic reticulum (ER), which catalyzes the formation and breakage of disulfide bonds in proteins. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.119 ng/mL, which can accurately detect low concentrations of PDIA2 in the sample.
Target PDIA2
N-Glycosylation Site 127, 284, 516
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.119 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Protein disulfide isomerase A2
Alternate Names PDIA2; Protein disulfide isomerase A2; PDA2, PDIR; Protein disulfide isomerase-associated 2; Pancreas-specific protein disulfide isomerase
Uniprot No. Q13087
Application The quantitative human PDIA2 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and biochemistry, particularly in studies of protein folding and ER stress. Researchers use it to measure PDIA2 levels in cells and tissues. The kit has applications in studies of protein folding and cellular stress responses.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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