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Quantitative Human PIEZO1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX714

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Specifications
Product Description
The quantitative human PIEZO1 (glycosylated) sandwich ELISA kit is designed to detect human piezo-type mechanosensitive ion channel component 1 (PIEZO1) levels. PIEZO1 is a protein that forms a mechanosensitive ion channel. This channel opens in response to mechanical stimuli. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.067 ng/mL, which can accurately detect low concentrations of PIEZO1 in the sample.
Target
PIEZO-1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.067 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Piezo-type mechanosensitive ion channel component 1
Alternate Names
PIEZO-1; Piezo-type mechanosensitive ion channel component 1; FAM38A
Uniprot No.
N-Glycosylation Site
295, 2294
Application Notes
Application
The quantitative human PIEZO-1 (glycosylated) sandwich ELISA kit provides a method for quantifying glycosylated PIEZO1 in biological samples. This measurement is valuable for research on mechanosensation and touch sensitivity. The kit also has potential applications in investigations of diseases related to mechanical sensing.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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