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Quantitative Human PTPRJ (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX554

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Specifications
Product Description
The quantitative human PTPRJ (glycosylated) sandwich ELISA kit is designed to detect human protein tyrosine phosphatase receptor type J (PTPRJ) levels. PTPRJ is a transmembrane protein that belongs to the protein tyrosine phosphatase (PTP) family. PTPRJ regulates various signaling pathways involved in cell growth, adhesion, and differentiation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of PTPRJ in the sample.
Target
PTPRJ
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.120 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Protein tyrosine phosphatase receptor type J
Alternate Names
PTPRJ; Protein tyrosine phosphatase receptor type J; CD148; PTPR-J; DEP1; Protein-tyrosine phosphatase eta; Density-enhanced phosphatase 1
Uniprot No.
N-Glycosylation Site
72, 82, 93, 104, 142, 172, 192, 231, 258, 278, 342, 351, 376, 391, 396, 413, 431, 501, 525, 536, 582, 603, 618, 628, 637, 666, 761, 772, 784, 790, 824, 910, 937
Application Notes
Application
The quantitative human PTPRJ (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and cancer biology, particularly in studies of cell signaling and tumor suppression. Researchers use it to measure PTPRJ levels in tissues and cell extracts. The kit has applications in studies of cancer and cell growth regulation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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