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Quantitative Human RAB5A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX285

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Specifications
Product Description
The quantitative human RAB5A (glycosylated) sandwich ELISA kit is designed to detect human raB5A, member ras oncogene family (RAB5A) levels. RAB5A is a small GTPase protein. This protein is involved in early endocytosis, which plays a role in the transport of molecules from the cell surface to endosomes. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.172 ng/mL, which can accurately detect low concentrations of RAB5A in the sample.
Target
RAB5A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.172 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.5 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
RaB5A
Alternate Names
RAB5A; RaB5A
Uniprot No.
N-Glycosylation Site
120
Application Notes
Application
The quantitative human RAB5A (glycosylated) sandwich ELISA kit provides a tool for quantitatively measuring glycosylated RAB5A levels in biological samples. It can be used in studies examining membrane trafficking, signal transduction, and cancer. The kit is applicable in cell biology research.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-90%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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