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Quantitative Human Rac1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX496)

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1×48 T
1×96 T

Product Description The quantitative human Rac1 (glycosylated) sandwich ELISA kit is designed to detect human ras related C3 botulinum toxin substrate 1 (Rac1) levels. Rac1 is a small GTPase protein that belongs to the Ras superfamily, which plays a crucial role in regulating various cellular processes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.112 ng/mL, which can accurately detect low concentrations of Rac1 in the sample.
Target Rac1
O-Glycosylation Site 32, 35
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.112 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Ras related C3 botulinum toxin substrate 1
Alternate Names Rac1; Ras related C3 botulinum toxin substrate 1; TC-25; MIG5; Cell migration-inducing gene 5 protein; Ras-like protein TC25
Uniprot No. P63000
Application The quantitative human Rac1 (glycosylated) sandwich ELISA kit is designed to measure glycosylated Rac1 levels in samples. This measurement is valuable in research related to cell signaling. The kit is applied in studies of cellular processes, which helps in understanding the role of Rac1.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-90%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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