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Quantitative Human SEMA3B (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX558)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SEMA3B (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 3B (SEMA3B) levels. SEMA3B is a secreted protein that belongs to the semaphorin family. Semaphorins are a large group of proteins that play a role in axon guidance and cell migration. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.063 ng/mL, which can accurately detect low concentrations of SEMA3B in the sample.
Target SEMA3B
N-Glycosylation Site 82, 124, 427
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.063 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Semaphorin 3B
Alternate Names SEMA3B; Semaphorin 3B; SEMA5; SemA; Semaphorin-V
Uniprot No. Q13214
Application The quantitative human SEMA3B (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and cancer biology, particularly in studies of axon guidance and tumor suppression. Researchers use it to measure SEMA3B levels in tissues. The kit has applications in studies of cancer and developmental biology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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