Quantitative Human SEMA3B (Glycosylated) Sandwich ELISA Kit

The quantitative human SEMA3B (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 3B (SEMA3B) levels. SEMA3B is a secreted protein that belongs to the semaphorin family. Semaphorins are a large group of proteins that play a role in axon guidance and cell migration. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.063 ng/mL, which can accurately detect low concentrations of SEMA3B in the sample.

SEMA3B

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.063 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Semaphorin 3B

SEMA3B; Semaphorin 3B; SEMA5; SemA; Semaphorin-V

82, 124, 427

The quantitative human SEMA3B (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and cancer biology, particularly in studies of axon guidance and tumor suppression. Researchers use it to measure SEMA3B levels in tissues. The kit has applications in studies of cancer and developmental biology.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.