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Quantitative Human SEMA5A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX572)

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1×48 T
1×96 T

Product Description The quantitative human SEMA5A (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 5A (SEMA5A) levels. SEMA5A is a transmembrane protein that belongs to the semaphorin family. SEMA5A plays a role in establishing neural connections, which also influences cell adhesion and migration in other tissues. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.352 ng/mL, which can accurately detect low concentrations of SEMA5A in the sample.
Target SEMA5A
N-Glycosylation Site 142, 168, 227, 277, 323, 367, 437, 536, 591, 717, 933
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.352 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.25 ng/mL-40 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Semaphorin 5A
Alternate Names SEMA5A; Semaphorin 5A; SEMAF; SemF; Semaphorin-F
Uniprot No. Q13591
Application The quantitative human SEMA5A (glycosylated) sandwich ELISA kit is a valuable tool for research in cell and developmental biology, particularly in studies of axon guidance. Researchers can measure SEMA5A levels in tissues and cells. Applications include studies of neurological development and cancer.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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