Quantitative Human SEMA5A (Glycosylated) Sandwich ELISA Kit

The quantitative human SEMA5A (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 5A (SEMA5A) levels. SEMA5A is a transmembrane protein that belongs to the semaphorin family. SEMA5A plays a role in establishing neural connections, which also influences cell adhesion and migration in other tissues. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.352 ng/mL, which can accurately detect low concentrations of SEMA5A in the sample.

SEMA5A

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.352 ng/mL

Quantitative sandwich ELISA

2.25 ng/mL-40 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Semaphorin 5A

SEMA5A; Semaphorin 5A; SEMAF; SemF; Semaphorin-F

142, 168, 227, 277, 323, 367, 437, 536, 591, 717, 933

The quantitative human SEMA5A (glycosylated) sandwich ELISA kit is a valuable tool for research in cell and developmental biology, particularly in studies of axon guidance. Researchers can measure SEMA5A levels in tissues and cells. Applications include studies of neurological development and cancer.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.