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Quantitative Human VAChT (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX614)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human VAChT (glycosylated) sandwich ELISA kit is designed to detect human vesicular acetylcholine transporter (VAChT) levels. VAChT is an integral membrane protein responsible for transporting acetylcholine into synaptic vesicles. This process is essential for neurotransmission at cholinergic synapses. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.124 ng/mL, which can accurately detect low concentrations of VAChT in the sample.
Target VAChT
N-Glycosylation Site 89, 96
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.124 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Vesicular acetylcholine transporter
Alternate Names VAChT; Vesicular acetylcholine transporter; SLC18A3; Solute carrier family 18 member 3, vesicular acetylcholine
Uniprot No. Q16572
Application The quantitative human VAChT (glycosylated) sandwich ELISA kit is designed to determine the concentration of this transporter protein in biological samples. The kit finds application in research related to neuroscience, neurotransmission, and cholinergic systems. It is valuable in studies investigating the role of acetylcholine in neurological disorders.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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