Quantitative Human VAChT (Glycosylated) Sandwich ELISA Kit

The quantitative human VAChT (glycosylated) sandwich ELISA kit is designed to detect human vesicular acetylcholine transporter (VAChT) levels. VAChT is an integral membrane protein responsible for transporting acetylcholine into synaptic vesicles. This process is essential for neurotransmission at cholinergic synapses. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.124 ng/mL, which can accurately detect low concentrations of VAChT in the sample.

VAChT

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.124 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Vesicular acetylcholine transporter

VAChT; Vesicular acetylcholine transporter; SLC18A3; Solute carrier family 18 member 3, vesicular acetylcholine

89, 96

The quantitative human VAChT (glycosylated) sandwich ELISA kit is designed to determine the concentration of this transporter protein in biological samples. The kit finds application in research related to neuroscience, neurotransmission, and cholinergic systems. It is valuable in studies investigating the role of acetylcholine in neurological disorders.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.