GlycoErase™ AMY2A Knockout HEK293T Cell Line

GlycoErase™ AMY2A knockout HEK293T cell line is a human embryonic kidney (HEK293T) model in which AMY2A expression has been eliminated. AMY2A encodes a pancreatic alpha-amylase that initiates starch and glycogen hydrolysis by cleaving 1,4-alpha-glucosidic bonds. In a human cellular background, loss of AMY2A enables mechanistic studies of secreted hydrolase biosynthesis, trafficking, and isoform-specific control of carbohydrate breakdown, supporting applications in metabolic disease modeling, digestive enzyme biology, and therapeutic enzyme engineering.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293T

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Penicillin/Streptomycin

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

AMY2A

Amylase Alpha 2A

PA; AMY2

1p21.1

This pancreatic amylase enzyme hydrolyzes starch and glycogen by cleaving 1,4-alpha-glucoside bonds, catalyzing the first step of digestion. It is part of a gene cluster on chromosome 1.