GlycoErase™ AMY2A Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF499)

Description	GlycoErase™ AMY2A knockout HEK293T cell line is a human embryonic kidney (HEK293T) model in which AMY2A expression has been eliminated. AMY2A encodes a pancreatic alpha-amylase that initiates starch and glycogen hydrolysis by cleaving 1,4-alpha-glucosidic bonds. In a human cellular background, loss of AMY2A enables mechanistic studies of secreted hydrolase biosynthesis, trafficking, and isoform-specific control of carbohydrate breakdown, supporting applications in metabolic disease modeling, digestive enzyme biology, and therapeutic enzyme engineering.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AMY2A
Full Name	Amylase Alpha 2A
Alternative Name	PA; AMY2
Location	1p21.1
Gene ID	279
Summary	This pancreatic amylase enzyme hydrolyzes starch and glycogen by cleaving 1,4-alpha-glucoside bonds, catalyzing the first step of digestion. It is part of a gene cluster on chromosome 1.