GlycoErase™ PRKAA1 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF702)

Description	GlycoErase™ PRKAA1 knockout HepG2 cell line is a HepG2 model lacking PRKAA1, the α1 catalytic subunit of AMPK, which senses cellular energy via AMP/ATP changes and regulates metabolic pathways. This knockout facilitates studies of energy-stress signaling, metabolic rewiring, and AMPK-dependent pathway pharmacology in liver metabolism.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PRKAA1
Full Name	Protein Kinase Amp-Activated Catalytic Subunit Alpha 1
Alternative Name	AMPK; AMPKa1; AMPK alpha 1
Location	5p13.1
Gene ID	5562
Summary	The encoded protein is the alpha catalytic chain of AMP-activated protein kinase (AMPK), which responds to increased AMP/ATP ratios by phosphorylating metabolic enzymes to conserve energy. Several isoforms arise from alternative transcripts.