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GlycoFlux™ AAV1-Human GCNT1 Viral Particle (CAT#: GLJF-1025-JF836)

Datasheet

SPECIFIC INQUIRY
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Description GlycoFlux™ AAV1-GCNT1 Viral Particle builds core-2 O-glycan branches by extending Galβ1-3(GlcNAcβ1-6)GalNAc, enabling mucin diversification and selectin-ligand tuning; AAV1 maintains high-level, long-lasting expression across retina, CNS, liver, heart, inner ear, pancreas, and smooth muscle to support adhesion-biology experiments.
Product Type Recombinant AAV1
Target GCNT1
Species Human
Promotor CMV, CAG, TRE, or other promoters
Tissue Tropism Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver
Packaging System AAV Triple-Plasmid Transfection System
Packaging Cell HEK293T
Application This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.
Shipping Shipped on dry ice, with temperature maintained at ≤ -80°C.
Storage Store at -80°C to preserve viral titer and stability.
Handling Notes Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.
Titer Assay qPCR/TCID50
Mycoplasma Test No mycoplasma contamination was detected.
Sterility Sterility testing verified the absence of microbial contamination.
Transduction Evaluation Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.
Full Name Glucosaminyl (N-acetyl) transferase 1
Alternative Name C2GNT; NACGT2; NAGCT2
Location 9q21.13
Gene ID 2650
Summary Essential for the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch, contributing to glycan diversity.
For Research Use Only.
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