GlycoFlux™ Human PPP1R12A Overexpression HEK293T Cell Line (CAT#: GLJF-0925-JF560)

Description	GlycoFlux™ human PPP1R12A overexpression HEK293T cell line is a stable cell line engineered to express MYPT1, the myosin phosphatase target subunit 1 that regulates actin-myosin interactions and smooth muscle contraction. MYPT1 directs PP1c to myosin substrates, influencing cytoskeletal remodeling, cell motility, and vascular tone. With several isoforms identified, this model supports research in smooth muscle physiology, contractility disorders, and cytoskeletal regulation.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R12A
Full Name	Protein Phosphatase 1 Regulatory Subunit 12A
Alternative Name	MBS; GUBS; M130; MYPT1
Location	12q21.2-q21.31
Gene ID	4659
Summary	Myosin phosphatase target subunit 1 (MYPT1) is a regulatory subunit of myosin phosphatase, involved in smooth muscle contraction and actin-myosin interactions. Several isoforms have been identified.